Journal: Journal of Ovarian Research

Article Title: Effect of testosterone on the Connexin37 of sexual mature mouse cumulus oocyte complex

doi: 10.1186/s13048-016-0290-3

Figure Lengend Snippet: Expression of Cx37 in COCs in different concentrations of testosterone. The expression of Cx37 which were treated with T was higher than that of blank control group. Cx37 increased with the elevated testosterone concentrations, and there was significantly difference in four groups of being cultured with testosterone

Article Snippet: After blocked with 5% skimmed milk for 1 h, the membranes were incubated with primary goat anti-mouse Cx37 antibody (1:200, Santa Cruz, USA) for 1 h at room temperature and overnight at 4 °C.

Techniques: Expressing, Control, Cell Culture

Journal: Journal of Ovarian Research

Article Title: Effect of testosterone on the Connexin37 of sexual mature mouse cumulus oocyte complex

doi: 10.1186/s13048-016-0290-3

Figure Lengend Snippet: Expression of Cx37 in COCs in androgen receptor antagonist. The expression of Cx37 which were treated with 10 −7 mol/L T and 10 −6 mol/L Flutamide was lower than that of treated with 10 −7 mol/L T. There were significant differences between two groups

Article Snippet: After blocked with 5% skimmed milk for 1 h, the membranes were incubated with primary goat anti-mouse Cx37 antibody (1:200, Santa Cruz, USA) for 1 h at room temperature and overnight at 4 °C.

Techniques: Expressing

Journal: Molecular medicine reports

Article Title: Connexin 43 expressed in endothelial cells modulates monocyte‑endothelial adhesion by regulating cell adhesion proteins.

doi: 10.3892/mmr.2015.4273

Figure Lengend Snippet: Figure 4. Altered expression of Cx43 reveals no affect on the expression levels of Cx37 and Cx40. The expression of Cx37 remained unchanged when (A) HUVEC were exposed to oleamide (50 µM) or retinoic acid (25 µM) for 24 h (n=4; *P<0.05) and (B) when the expression of Cx43 was altered by transfec tion with pcDNA‑Cx43 or by siRNA‑Cx43 (n=4; *P<0.05). The expression of Cx40 remained unchanged when (C) HUVEC were exposed to oleamide (50 µM) or retinoic acid (25 µM) for 24 h (n=4; *P<0.05) and (D) when Cx43 expression was altered by transfection with pcDNA‑Cx43 or by siRNA‑Cx43 (n=4; *P<0.05). HUVEC; human umbilical vein endothelial cells; Cx, connexin; si, small interfering.

Article Snippet: Secondary antibodies of Cx37 (1:2,000; donkey anti-goat IgG-HRP; cat. no. sc-2020; Santa Cruz Biotechnology, Inc.) was used at 1:1,000.

Techniques: Expressing, Transfection

Journal: American Journal of Physiology - Cell Physiology

Article Title: Mitochondrial connexin40 regulates mitochondrial calcium uptake in coronary endothelial cells

doi: 10.1152/ajpcell.00283.2016

Figure Lengend Snippet: Protein levels of Cx40, Cx37, Cx43, and Cx45 in MCECs. A and D: typical WB images of Cx40, Cx37, Cx43, and Cx45 protein levels in the whole lysate of MCECs isolated from WT, Cx40 KO, and Cx37 KO mice. Actin was used as a loading control for samples of whole lysate from MCECs. B: Cx40 protein level normalized to actin: WT (n = 5) and Cx40 KO (n = 5). Data are means ± SE. C: Cx37 protein levels in MCECs of WT and Cx40 KO mice (n = 4 in each group). Data are means ± SE. E: Cx43 protein levels in MCECs of WT and Cx40 KO mice (n = 5 in each group). Data are means ± SE. F: Cx45 protein levels in MCECs of WT and Cx40 KO mice (n = 6 in each group). Data are means ± SE. G: photomicrographs show typical image of Cx40 expression in WT MCECs (left) and Cx40 KO MCECs (right). MCECs were stained with Cx40 (green) and Tom20 (a marker of mitochondria; red).

Article Snippet: Blots were incubated with the following primary antibodies: anti-Cx40 (Santa Cruz, sc-20466; 1:2,000), anti-ATP5A (Santa Cruz, sc-136178; 1:2,000), anti-Actin (Santa Cruz, sc-1616; 1:4,000), anti-Cx37 (Thermo Fisher Scientific, 42-4400; 1:1,000), anti-Cx43 (Thermo Fisher Scientific, 71-0700; 1:2,000), anti-Cx45 (Thermo Fisher Scientific, 41-5800; 1:2,000), or anti-GAPDH (Thermo Fisher Scientific, MA5-15738; 1:4,000), followed by horseradish peroxidase-linked secondary antibody incubation.

Techniques: Isolation, Expressing, Staining, Marker

Journal: Molecular reproduction and development

Article Title: Role of focal adhesion kinase in oocyte-follicle communication

doi: 10.1002/mrd.22446

Figure Lengend Snippet: Effect of ptk2 ablation on the frequency of transzonal processes and connexin plaques. COCs containing ptk2-null (A,C,E) or WT (B,D,F) oocytes were labeled with either rhodamine-phalloidin (A, B) to detect the filamentous actin core (arrows) of transzonal processes (white); with anti-connexin 43 followed by alexa565—goat anti-rabbit (C,D) where connexin 43 foci (arrows) are labeled red ; or with anti- connexin 37 followed by alexa488-goat anti- rabbit (E,F) where connexin 37 foci (arrows) labeled green.

Article Snippet: Antibodies used included anti-PTK2 C20 (#sc-558), anti-connexin-43 (sc-9059; Santa Cruz, Santa Cruz, CA, USA), anti-connexin 37 (Invitrogen, Carlsbad, CA USA) activated PTK2 pY 861 (BioSource #44-652, Invitrogen, Grand Island, NY, USA).

Techniques: Labeling

Journal:

Article Title: Reduction of electrical coupling between microvascular endothelial cells by NO depends on connexin37

doi: 10.1152/ajpheart.01148.2008

Figure Lengend Snippet: Effect of DETA exposure on intercellular resistance in wild-type, connexin (Cx) 37 null, Cx40 null, and Cx43G60S (nonfunctional mutant) MMEC. DETA (500 μM, 3 h) increased resistance in cells derived from wild-type, Cx40 null, and Cx43G60S mice, but not in cells from Cx37 null mice. *Significant difference from the appropriate control group, P < 0.05; n = 16 in group 1 (pooled from all control experiments), n = 21 in group 2 (pooled), and n = 4 in groups 3–8.

Article Snippet: Peroxidase-labeled anti-rabbit IgG antibodies for anti-Cx37, anti-PKG, and anti-phosphoserine antibodies were purchased from Biolynx (Brockville, ON) and Cell Signaling Technology (Beverly, MA).

Techniques: Mutagenesis, Derivative Assay

Journal:

Article Title: Reduction of electrical coupling between microvascular endothelial cells by NO depends on connexin37

doi: 10.1152/ajpheart.01148.2008

Figure Lengend Snippet: Lack of effect of DETA on Cx37, Cx40, and Cx43 protein expression in wild-type MMEC. A: Cx37, Cx40, Cx43, and β-actin immunoblots from control and DETA-treated MMEC (500 μM, 3 h). B: densitometric ratios of Cx37, Cx40, and Cx43 to β-actin immunoblots for control and DETA-treated MMEC. DETA had no effect on Cx37, Cx40, or Cx43 protein expression; n = 3 in each group.

Article Snippet: Peroxidase-labeled anti-rabbit IgG antibodies for anti-Cx37, anti-PKG, and anti-phosphoserine antibodies were purchased from Biolynx (Brockville, ON) and Cell Signaling Technology (Beverly, MA).

Techniques: Expressing, Western Blot

Journal:

Article Title: Reduction of electrical coupling between microvascular endothelial cells by NO depends on connexin37

doi: 10.1152/ajpheart.01148.2008

Figure Lengend Snippet: Lack of effect of DETA on distribution of Cx37, Cx40, and Cx43 within wild-type MMEC. Top: representative examples of control (n = 9) and DETA-treated cells (500 μM, 3 h, n = 6) probed for Cx37. Although most of the immunoreactivity is cytoplasmic, occasional punctate staining (arrows) indicates putative Cx37 gap junctions. Comparable staining was also seen with another anti-Cx37 antibody (i.e., that used for immunoblotting) (data not shown). The superimposed oval uniform gray shapes are cell nuclei identified with Hoechst 33258 stain. Middle: representative examples of control (n = 7) and DETA-treated (n = 7) cells probed for Cx40. Numerous small Cx40 gap junctions are indicated by the punctate staining. The superimposed oval gray shapes are cell nuclei. Bottom: representative examples of control (n = 3) and DETA-treated (n = 3) cells probed for Cx43. Numerous Cx43 gap junctions of various sizes are evident, larger than those containing Cx37 or Cx40, whereas gray ovals indicate cell nuclei. Control experiments where the connexin primary antibodies were omitted showed only gray oval nuclei (data not shown). Bar at bottom right indicates 20-μm scale for all panels.

Article Snippet: Peroxidase-labeled anti-rabbit IgG antibodies for anti-Cx37, anti-PKG, and anti-phosphoserine antibodies were purchased from Biolynx (Brockville, ON) and Cell Signaling Technology (Beverly, MA).

Techniques: Staining, Western Blot